Inclusion of chia seeds (Salvia hispanica L.) and pumpkin seeds (Cucurbita moschata) in dairy sheep diets.

Chia (Salvia hispanica L.) seed (CS) and Pumpkin (Cucurbita moschata) seed (PS) are used in ruminant diets as energy sources. The current experiment studied the impact of dietary inclusion of CS and PS on nutrient intake and digestibility, milk yield, and milk composition of dairy sheep. Twelve primiparous Texel × Suffolk ewes [70 ± 5 days in milk (DIM); 0.320 ± 0.029 kg milk yield] were distributed in a 4 × 3 Latin square design and fed either a butter-based control diet [CON; 13 g/kg dry matter] or two diets with 61 g/kg DM of either CS or PS. Dietary inclusion of CS and PS did not alter live weight (p >0.1) and DM intake (p >0.1). However, compared to the CON, dietary inclusion of both CS and PS increased the digestibility of neutral detergent fiber (p <0.001) and acid detergent lignin (p < 0.001). Milk production (p = 0.001), fat-corrected milk (p < 0.001), and feed efficiency (p < 0.001) were enhanced with PS, while the highest milk protein yield (p < 0.05) and lactose yield (p < 0.001) were for CS-fed ewes. Compared to the CON diet, the ingestion of either CS and/or PS decreased (p < 0.001) the C16:0 in milk. Moreover, both CS and PS tended to enhance the content of C18:3n6 (p > 0.05) and C18:3n3 (p > 0.05). Overall short-term feeding of CS and/or PS (up to 6.1% DM of diet) not only maintains the production performance and digestibility of nutrients but also positively modifies the milk FA composition.

Effect of replacing soybean meal (Glycine max) with sesame meal (Sesamum indicum) on productive traits, carcass characteristics, and gross profit margin in fattening lamb's diets.

The objective of this study was to determine the effect of replacing soybean meal (Glycine max) with sesame meal (Sesamum indicum) on productive traits, carcass characteristics, and gross profit margin (GMP) in fattening lamb's diets. For this, 42 Katahdin lambs were divided into three treatments in duplicate: basal diet + soybean meal (100S), basal diet + sesame meal/soybean meal (50/50SA), and basal diet + sesame meal (100A). Dry matter intake, daily weight gain (DWG), total kg gained (KgT), feed conversion (FC), and feed efficiency (FE) were evaluated; upon reaching the weight for sale, the animals were slaughtered, and hot carcass weight (HCW) was evaluated. The results were analyzed with a completely randomized design with repeated measures. Regarding time, no differences were found between treatments, for DWG (0.171 ± 0.006 kg/d), FC (6.7 ± 0.55), FE (0.175 ± 0.02), KgT (2.86 ± 0.13 kg), HCW (50.97 ± 0.79 kg), as well as for chest depth (26.96 ± 0.33 cm), leg width (20.63 ± 0.028 cm), leg diameter (60.7 ± 0.44 cm), and ribs width (24.05 ± 0.14 cm). GPM was 16.50%, 18.63%, and 19.97% for 100S, 50/50SA, and 100A, respectively. Overall, in fatting lamb diets, replacing soybean meal with sesame meal by either 50% or 100% substitution could be a feasible feeding strategy as in both cases, gross profit was increased, and no negative effects were found for productive traits and carcass quality.

Effect of different dietary inclusion levels of whole plant green tomato (Physalis philadelphica) silage on nutrient intake and digestibility, and in vitro rumen fermentation kinetics in sheep.

Mexico has many agricultural by-products that can be used for animal feed, and green tomatoes are produced throughout the country and can be an alternative to overcome the high prices of cereal-based feeds. This study determined in vitro fermentation kinetics, production performance, nutrient intake, digestibility, and nitrogen balance from sheep supplemented with whole plant green tomato (GT) on corn silage (CS) based diets. For 21 days, eighteen Suffolk lambs (38 ± 4 kg of live weight) were grouped into three dietary GT inclusion levels to replace CS: a control diet based on 100% CS (GT0, 570 g /kg dry matter, DM), while 100 g/kg DM (GT100) and 200 g/kg DM (GT200) of GT were included as a replacement for CS. A completely randomized design was used to measure in vitro gas production, in vitro rumen fermentation, chemical composition, and in vivo parameters. In vitro gas production, "A" (ml/g DM), fermentation rates "B," (h-1), and "C" (h-½), were lower for GT200, while DM disappearance (mg/100mg) was lower for GT100 compared with GT0. Compared to GT0, GT100 and GT200 did not affect (P > 0.05) DM and organic matter (OM) intake (g/kgLW0.75). Ether extract intake was higher for GT0 and GT100 (P < 0.001) compared to GT200. Neutral detergent fiber (NDF) intake was higher (P < 0.05) for GT200 compared with GT0. Intake of lignin was higher (P < 0.001) for GT200 than that of GT0 and GT100. Digestibility coefficients for DM, OM, NDF, and Acid detergent fiber (ADF) were lower (P < 0.05) in GT100 than in the rest of the treatments. Nitrogen intake and N excreted in feces and urine were lower (P < 0.001) for GT0. N balance was negative for all treatments, being higher for GT200 (P < 0.05). Overall, the addition of GT at 100 or 200 g/kg DM in sheep diets negatively affects nutrient digestibility and N balance, so their dietary inclusion is not recommended.

Effect of the Inclusion of Bacillus spp. in Growing-Finishing Pigs' Diets: A Meta-Analysis.

This meta-analysis determined the effect of Bacillus spp. on growth performance of growing−finishing pigs and then assessed causes for the heterogeneity of responses detected using meta-regression. A database of 22 articles published from 2000 to 2020 was identified, and 9 articles fitted the selection criteria and were integrated in the final database. Statistical analysis was performed to analyze the effect size for ADG, average daily feed intake (ADFI), and F:G ratio using a standardized means difference (SMD) at a 95% confidence interval. A meta-regression analysis was used to investigate the cause of heterogeneity, using the individual SMD for each study assessment as the outcome and the associated SE as the measure of variance. Dietary Bacillus spp. supplementation had no effect on ADFI (SMD: −0.052, p = 0.138) and numerically increased ADG (SMD: 0.113, p = 0.081) and reduced the F:G ratio SMD: −0.127, p < 0.001). Meta-regression outcomes suggested that the number of animals per group was an essential component promoting heterogeneity in ADG. Overall, the inclusion of Bacillus spp. (median 486 mg/d) in growing−finishing pigs can increase ADG and can decrease the F:G ratio.

Influence of Mediterranean climate and lunar calendar on milk production in Lacaune breed ewes.

Environmental factors affect daily milk production in dairy animals. The aim of this study was to quantify the effects of environmental factors, specifically mean temperature (°C), relative humidity (%), temperature-humidity index (THI), solar radiation (°), pluvial precipitation (mm) and lunar calendar (full moon, waxing quarter, waning quarter, new moon), on milk production (kg/d). The analysis was based on 96,195 morning and evening milking records documented on 109 consecutive days, from 869 Lacaune ewes. Ewes were housed in groups of 174 individuals. The analysis was performed in two independent procedures, a Pearson correlation analysis and a multivariate analysis of the ewe's interrelationships, which was based on the total variance estimate and a Varimax-rotated factorial analysis. Milk yield (kg/d) was significantly (p < 0.05) negatively correlated with mean temperature (-0.24), relative humidity (-0.16), THI (-0.24), and radiation (-0.18), which suggests that the higher these environmental factors, the lower the milk yield. Lunar calendar had a significant (p < 0.01) effect on milk production yield; specifically, yields were higher on the full moon and new moon (2.25 ± 0.05 kg/day) than they were on the crescent or waning moon (2.17 ± 0.05 kg/day). In conclusion, ewes that had been exposed to higher mean temperature, relative humidity, THI and solar radiation had the lowest milk yield, and milk yields are highest on full and new moons. The results of this work may be helpful in making predictions for milk production in Lacaune ewes in the Mediterranean region throughout the year.

Insects as alternative feed for ruminants: comparison of protein evaluation methods.

BACKGROUND: The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich feeds. The use of insects seems promising, but the extent of their ruminal protein degradation is largely unknown. This parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant farming. In addition, although assessing ruminal N degradation represents a key first step to examine the potential of new feeds, it is a challenging task due to the lack of a reference method. This study was conducted to investigate the potential of 4 insects (Tenebrio molitor, Zophobas morio, Alphitobius diaperinus and Acheta domesticus) as alternative protein sources for ruminants, using 3 methodologies: 1) a regression technique based on the in vitro relationship between gas production and ammonia-N concentration; 2) a conventional in vitro technique of batch cultures of ruminal microorganisms, based on filtering the incubation residue through sintered glass crucibles; and 3) the in situ nylon bag technique. The in vitro intestinal digestibility of the non-degraded protein in the rumen was also determined. Soybean meal was used as a reference feedstuff. RESULTS: Comparison of evaluation methods (regression, in vitro and in situ) did not allow to reliably select a single value of ruminal N degradation for the studied substrates, but all techniques seem to establish a similar ranking, with good correlations between methods, particularly between regression and in situ results. Regardless of the methodology, nitrogen from the 4 insects (with contents ranging from 81 to 112 g/kg of dry matter) did not show high ruminal degradation (41-76%), this value being always lower than that of soybean meal. Furthermore, the in vitro intestinal digestibility of non-degraded N was relatively high in all feeds (≥ 64%). CONCLUSION: Overall, these results support the potential of the 4 studied insects as alternative feedstuffs for ruminants. Among them, T. molitor showed the lowest and greatest values of ruminal N degradation and intestinal digestibility, respectively, which would place it as probably the best option to replace dietary soybean meal and increase the sustainability of ruminant feeding.

A systematic-review on the role of exogenous enzymes on the productive performance at weaning, growing and finishing in pigs.

Supplementing exogenous enzymes in pig diets is an alternative solution to increase dietary energy and fiber digestibility to improve pig production performance at a low production cost and to reduce environmental impact with lower N and P excretions. The production stage, diet composition, enzyme source, amount and number of enzymes added, are factors to consider before using them. A database composed by 227 individual diets, resulting from 43 studies with 48 experimental records were divided in different production stages, with 19 records for weaning, 17 records for growing and 12 records for finishing. A descriptive statistical analysis of the chemical composition of the diets and enzyme doses was carried out. The data with normal distribution were analyzed calculating the mean, the minimum and maximum length, the standard deviation and the coefficient of variation. It was found that combined enzymes are the most widely reported enzyme combination in the supplementation of pigs at all stages of production. Phytases and Mannanases are commonly used at weaning and growing stages. Xylanases and Proteases have been reported to be used in all production stages. However, the highest yielding enzymes at weaning, growing and finishing stages were Phytases and Mannanases. Dietary supplementation of exogenous enzymes improves production characteristics at all stages of production. However, an improvement in growth performance and nutrient digestibility is not always observed. Future studies should focus on the interaction between production stages, composition of the diet, origin of the enzyme and the amount and number of enzymes added.

Feeding Forage Mixtures of Ryegrass (Lolium spp.) with Clover (Trifolium spp.) Supplemented with Local Feed Diets to Reduce Enteric Methane Emission Efficiency in Small-Scale Dairy Systems: A Simulated Study.

In cattle, greenhouse gas (GHG) emissions and nutrient balance are influenced by factors such as diet composition, intake, and digestibility. This study evaluated CH4 emissions and surpluses of crude protein, using five simulated scenarios of supplementation in small-scale dairy systems (SSDS). In addition, two pasture managements (cut-and-carry versus grazing) and two varieties of legumes (red clover vs. white clover) were considered. The diets were tested considering similar milk yield and chemical composition; CH4 emission was estimated using Tier-2 methodology from the Intergovernmental Panel on Climate Change (IPCC), and the data were analyzed in a completely randomized 5 × 2 × 2 factorial design. Differences (p < 0.05) were found in predicted CH4 emissions per kg of milk produced (g kg-1 FCM 3.5%). The lowest predicted CH4 emissions were found for S3 and S4 as well as for pastures containing white clover. Lower dietary surpluses of CP (p < 0.05) were observed for the control diet (1320 g CP/d), followed by S5 (1793 g CP/d), compared with S2 (2175 g CP/d), as well as in cut-and-carry management with red clover. A significant correlation (p < 0.001) was observed between dry matter intake and CH4 emissions (g-1 and per kg of milk produced). It is concluded that the environmental impact of formulating diets from local inputs (S3 and S4) can be reduced by making them more efficient in terms of methane kg-1 of milk in SSDS.

Worldwide Traceability of Antibiotic Residues from Livestock in Wastewater and Soil: A Systematic Review.

The use of antibiotics in animal production are widely used for disease treatment, health protection, and as growth promoters. Common antibiotics used in veterinary medicine are excreted and eliminated through the sewage system, contaminating water and soil with negative effects on agricultural activities. This systematic review focuses on the trend of research works on antibiotic residues, evaluating antibiotics used in livestock production and their excretion in animal products and in environmental matrices such as water and soil. Our database was composed of 165 articles, reporting the concentration of antibiotic residues found in the environment, livestock (cow, sheep, pig, horse, chicken, rabbit, goat), aquatic and terrestrial animal tissues, animal products (milk and eggs), wastewater, and soil. The documents were obtained from Asia, Africa, North America, South America, Europe, and Oceania. A descriptive analysis of antibiotic residues found worldwide was analyzed according to each of the variables used such as antibiotic family, name, concentration (% and mg/kg or ppm), and country and continent where the residue was found. The descriptive analysis was carried out using the "describe" function of psych package and pirate plots were drawn. According to our study, the main antibiotics used worldwide in animal production are sulfonamides, tetracyclines, quinolones, penicillin, and cephalosporins. At present, despite the trends of increased regulations on the use of antibiotics worldwide, antibiotics are still utilized in food animal production, and are present in water and soil, then, there is still the misuse of antibiotics in many countries. We need to become aware that antibiotic contamination is a global problem, and we are challenged to reduce and improve their use.

Production Performance, Nutrient Digestibility, and Milk Composition of Dairy Ewes Supplemented with Crushed Sunflower Seeds and Sunflower Seed Silage in Corn Silage-Based Diets.

This study determined production performance, nutrient digestibility, and milk composition of dairy ewes supplemented with crushed sunflower seeds (Helianthus annuus) and sunflower seed silage in corn silage-based diets. Six ewes were grouped in a double 3 × 3 Latin square design with three periods of 21 days. All treatments were based on ad libitum corn silage. Control diet was based on alfalfa hay (333 g/kg DM), sorghum grain (253 g/kg DM), triticale grain (200 g/kg DM), soybean meal (167 g /kg DM), and vitamin and mineral premix (47 g/kg DM). Sunflower seeds (SF) and sunflower seed silage (SFS) treatments consisted of alfalfa hay (333 g/kg DM), sorghum grain (267 g/kg DM), triticale grain (100 g/kg DM), soybean meal (167 g /kg DM), SF or SFS (87 g/kg DM) and vitamin and mineral premix (47 g/kg DM). Compared to control, SF and SFS increased intake and digestibility of fiber components, such as neutral detergent fiber (NDF) and acid detergent fiber (ADF). Body weight, nitrogen balance, milk yield, milk fat yield, milk protein yield, lactose yield and milk urea N were similar between treatments. Overall, results demonstrated that crushed sunflower seeds and ensiled seeds do not change significantly productive parameters of dairy sheep.

Milk Production of Lacaune Sheep with Different Degrees of Crossing with Manchega Sheep in a Commercial Flock in Spain.

The objective of the present study was to evaluate the effect of the grade of crossbreeding (Lacaune x Manchega) and environmental factors on milk production in a commercial flock in Spain. A total of 5769 milk production records of sheep with different degrees of purity of the Lacaune breed crossed with Manchega were used as follows: 100% Lacaune (n = 2960), 7/8 Lacaune (n = 502), 13/16 Lacaune (n = 306), 3/4 (n = 1288), 5/8 Lacaune (n = 441) and 1/2 Lacaune: Manchega (n = 272). Additional available information included the number of parity (1 to 8), litter size (single or multiple), and the season of the year of lambing (spring, summer, autumn and winter). A mixed model was used to evaluate the level of crossbreeding and environmental factors on milk production. The 100% Lacaune sheep presented the highest milk production with respect to the F1 Lacaune x Manchega sheep (p < 0.01), showing that as the degree of gene absorption increases with the Manchega breed, it presents lower milk yield. The 100%, 13/16, and 3/4 Lacaune genotypes had the highest milk yields with respect to the 1/2 Lacaune/Manchega breed (p < 0.001). The Lacaune registered on average 181.1 L in a period adjusted to 160 days of lactation (1.13 L/ day). Likewise, the parity number, litter size, and season of lambing effects showed significant differences (p < 0.01). It was concluded that 13/16 and 3/4 Lacaune/Manchega ewes presented the highest milk yields with respect to the other crosses.

Productive behavior in growing kid goats and methane production with the inclusion of chokecherry leaf (Prunus salicifolia).

Currently for the reduction of methane (CH4) emissions are using fodder rich in condensed tannins, however, not yet known exactly how they act in the rumen is not yet clear. The objective of this study was to evaluate the effect of the inclusion of leaves of Prunus salicifolia (PS, 0%, 15%, and 30%) on the productive behavior of growing kid goats, methane production, nutritional value, fermentation, and ruminal digestibility through an in vivo and in vitro study was performed. Diets were administered ad libitum to 6 Saanen kids with live weight (LW) of 12.25 ± 2.25 kg. Three levels of inclusion were used in a diet based on ground corn grain, soybean meal, corn stover, and oat hay in substitution with P. salicifolia leaves. The in vivo productive behavior was determined, as well as the fermentation kinetics, in vitro gas production, CH4 and hydrogen (H2) in an in vitro system was determined. For the in vivo study, we used a 3 × 3 Latin square design in in vivo study and an analysis of variance with three replications for in vitro gas production. The inclusion of 30% increased (P = 0.0011) dry matter intake (DMI 589.33 g/day) compared to the control group (418.80 g/day). The highest N excretion (feces and urine) (P < 0.001) was for T0, in addition to presenting a negative nitrogen balance compared with T15 and T30. The production of CH4 (mM)/g DM incubated, and CH4 (mM)/g DM fermented, CH4 (mM/day), and H2 was lower (P < 0.05) in T30 than T0 and T15. CH4 (mM)/day was lower (P < 0.036) in T130 (283 mM/day) compared with T0 (407 mM/day) P. salicifolia is a forage that helps to reduce the production of methane and can be included in the diets of growing kid goats in amounts less than 30% without affecting production performance.